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In current study, we considered the anti-cancer potential of TLBZT, applied alone or in combination with low dose of 5 Fluorouracil, in CT26 colon carcinoma in vivo. Methods. CT26 colon carcinoma was established in rats and handled with TLBZT, 5 Fu, or TLBZT plus 5 Fu. The tumor volumes were seen. Apoptosis was detected by buy GM6001 TUNEL assay. Caspases activities were detected by colorimetric assay. Mobile senescence was indentified by senescence B galactosidase staining. Angiogenesis and gene expression was seen by immunohistochemistry or western blot. Effects. TLBZT somewhat restricted CT26 colon carcinoma development. TLBZT elicited apoptosis in CT26 colon carcinoma, combined with Caspase 3, 8, and 9 activation and PARP cleavage, and downregulation of Survivin and XIAP. TLBZT also induced mobile senescence in CT26 colon carcinoma, with concomitant up-regulation of p21 and p16 and downregulation of RB phosphorylation. In addition, angiogenesis and VEGF expression in CT26 colon carcinoma was notably inhibited by TLBZT therapy. Moreover, TLBZT Ribonucleic acid (RNA) significantly enhanced anti-cancer effects of 5 Fu in CT26 colon carcinoma. Results. TLBZT displayed dramatically anticancer effect, and increased the effects of 5 Fu in CT26 colon carcinoma, which might correlate with induction of apoptosis and cell senescence, and angiogenesis inhibition. The present study offers new information in to TCM approaches for colon cancer treatment which might be worth of further study. Key words. Colon carcinoma, Chinese herbal formula, Apoptosis, Cell senescence, Angiogenesis Correspondence. 1Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, order 3-Deazaneplanocin A Shanghai 200032, PR China 2Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, PR China Full list of author information can be acquired at the end-of the. licensee BioMed Central Ltd. This is an Open Access report distributed under the conditions of the Creative Commons Attribution License, which allows infinite use, distribution, and reproduction in any medium, offered the original work is precisely cited. Deng et al. BMC Complementary and Alternative Medicine 2013, 13. 128 Page 2 of 11Background Colorectal cancer is the 3rd most commonly diagnosed cancer in males and the second in females worldwide. The incidence of colorectal cancer is increasing as a result of overweight, absence regular activities, smoking and obesity, red and processed meat consumption, and excessive al cohol consumption. The current treatment of colo-rectal cancer mainly depends upon radiotherapy, chemotherapy, surgery and targeted therapy. But, the preventive influence of these treatments are less than satisfactory, the 5 year over all survival after resection for colon cancer is about 60%, the 5 year survival for metastatic colorec tal cancer is approximately 10%.